Which factor is NOT a cause of hemolysis during specimen collection?

The correct response identifies that using a proper syringe is not a cause of hemolysis during specimen collection. When collecting blood samples, utilizing the appropriate syringe ensures that the sample is drawn correctly and gently, which minimizes the risk of damaging the red blood cells. Properly designed syringes incorporate features that reduce the likelihood of hemolysis, such as appropriate internal smoothness and size, facilitating smooth blood flow without creating excessive turbulence.

In contrast, the other factors mentioned can lead to hemolysis. Vigorous shaking can disrupt red blood cells due to mechanical trauma, while extreme temperatures can affect their integrity. Centrifuging before clot formation can lead to the release of hemoglobin from lysed cells, further contributing to hemolysis. Understanding these principles is crucial for maintaining the quality of blood specimens.